Studying Neutrophil Function in vitro: Cell Models and Environmental Factors

Neutrophils are the most abundant immune cell type in blood and constitute first line of defense against invading pathogens. Despite their important role many diseases, they challenging to study due short life span inability cryopreserve or expand them vitro. Thus, research into neutrophils has rely on cells freshly isolated from peripheral human donors, introducing donor-dependent variation experimental data. To counteract these problems, researchers tried develop adequate models, such as lines. For those functional studies that cannot a standardization protocols regarding neutrophil purification culturing could be solution. In this review, we provide an overview commonly used models for function (HL-60, PLB-985, NB4, Kasumi-1 induced pluripotent stem cells). addition, describe effects glucose concentration, pH, oxygen tension temperature function.